Wet-Workshop for Flow Cytometry on Human B & T Cell Subset.

         

A wet-workshop for flow cytometry on human B & T cells will be held on November 3^rd – 4^th, 2009 in the beautiful city of Fayoum, Egypt. The purpose of this workshop is to interface experts in flow cytometry with people who have interest in flow cytometry and routinely use it in their research or clinical practice. This workshop will cover the basics of flow cytometry in research and clinical applications. This workshop will give a platform for hands-on training, gaining advance knowledge and discussion of application and difficulties and new ideas. In the workshop you will learn how to phenotype the subsets of: CD4 T cells (naive, memory, activated [early, long], regulatory) CD8 T cells (naive, central memory, effector memory, short-term effector, activated [early, long]) B cells (naive, pre-switch memory, post-switch memory, plasma, transitional, activated [early, long]) Innate cells (granulocytes, macrophages [tissue, circulating], activated).

• Speakers:
Prof. Margarida Carneiro
Head of Autoimmunity Group
Center for Neurosciences and Cell Biology
University of Coimbra
mmargarida.carneiro@gmail.com

• COURSE CONTENTS:

1^st Day: Tuesday November3^rd 2009Session 1: Topics in Basic Immunology:

09:00-10:30	Introduction to Flow Cytometry. * How to choose the color combinations (lasers, filters,fluorochromes) * Compensation * Flow cytometry beyond cell phenotyping
10:30 – 10:45	Short break
10:45 – 12:30	Sample collection and preparation
12:30 – 01:30	Lunch break
01:30 – 02:45	Washing and fixation of samples
02:45 – 03:00	Short break
03:00 – 06:00	Cytometer setup (compensation) and sample acquisition

2^nd Day:Wednesday November 4^gth 2009

9:00 - 10:30	Data analysis and discussion
10:30 – 10:45	Short break
10:45 – 12:30	Data analysis and discussion
12:30 – 01:30	Lunch and good-bye