Wet–Workshop on Real Time PCR in Immunogenetics
Fayoum University, Teaching Hospital, Fayoum, Egypt
  
• Objectives and Aims:
The purpose of this workshop is to interface experts in immunogenetics with people who have interest in immunogenetics and routinely use it in their research or clinical practice. This workshop will cover the basics of real time PCR in research and clinical applications. The program for the workshop is entirely determined by the interests of the attendees and the active participation.
• Workshop Director:
Prof: Paulo Santos; Head of Functional Genomics Laboratory, Portgal
• Workshop Agenda:
First day: Monday 2ndNovember 2009
Session 1: Theoretical Lectures Topics (L)
09:00-10:00 Real-time PCR: principles and applications (L)
10:00-11:00 Quantification strategies and Optimization (L)
11:00-11:30 Coffee break
11:30-12:30 Applications: infection, immunology and oncology (L)
Session
2: Practical Topics (P)
12:30-01:30 Sample processing and nucleic acid extraction
01:30-02:30 Lunch breach during RT PCR
02:30-05:00 qPCR set-up: current protocols
Second day: Tuesday 3rdNovember 2009
Session 1: Theoretical Lectures Topics (L)
09:00-10:00 Practical examples: gene expression,
high-resolution melting and allelic discrimination
10:00-11:00 Experimental design
11:00-11:30 Coffee break
11:30-12:30 Data analysis
Session 2: Practical Topics (P)
12:30-03:00 Validation and data analysis (Gene
expression)
2:00-3:00 lunch break during Data Analysis
03:00-04:00 (High-resolution melting and Allelic
discrimination )
• Participants:
Participation in wet labs, tutorials and other events will be limited to
registered students/participants. The wet labs will be open to 20 registered
students selected by the committee.
• Course Certificates:
Participants who attend the wet lab will receive a course completion
certificate.
• REGISTRATION AND TUTION FEES:
Government Research Institutions/Universities: 400 LE
Registration includes workshop kit, access to lectures and wet lab, morning tea,
working lunch and evening tea.
• ACCOMMODATION:
Participants will have to make their own living arrangements. However, reasonably priced hotel rooms have been blocked out for your convenience. For more details and assistance please contact info@faisoc.com
• HOW TO APPLY:
Please log on to our website www.faisoc.com. Go to
Preconference workshops and download the application form for registration.
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